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AP181P

Sigma-Aldrich

Goat Anti-Mouse IgG Antibody, HRP conjugate, Species Adsorbed

0.8 mg/mL, Chemicon®

Synonim(y):

HRP-Conjugated Goat Anti-Mouse IgG

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About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.46

pochodzenie biologiczne

goat

Poziom jakości

białko sprzężone

peroxidase conjugate

forma przeciwciała

F(ab′)2 fragment of affinity isolated antibody

rodzaj przeciwciała

secondary antibodies

klon

polyclonal

reaktywność gatunkowa

mouse

producent / nazwa handlowa

Chemicon®

stężenie

0.8 mg/mL

metody

ELISA: suitable
western blot: suitable

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

Powiązane kategorie

Opis ogólny

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Zastosowanie

EIA and Western blots: 1:5,000-1:100,000.

Immunohistochemistry: 1:500-1:5,000.

Optimal working dilutions must be determined by end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
This Goat anti-Mouse IgG Antibody, HRP conjugate, Species Adsorbed is validated for use in ELISA, WB for the detection of Mouse IgG.

Postać fizyczna

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25M NaCl, pH 7.6 with 15 mg/mL BSA.

RECONSTITUTION:

Reconstitute with 500 μL of sterile distilled water.

Przechowywanie i stabilność

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

WARNING:

Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Kod klasy składowania

13 - Non Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Odwiedź Bibliotekę dokumentów

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British Journal of Cancer null
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