Przejdź do zawartości
Merck
Wszystkie zdjęcia(2)

Kluczowe dokumenty

AB5054

Sigma-Aldrich

Anti-Calretinin Antibody

serum, Chemicon®

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych


About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702

pochodzenie biologiczne

rabbit

Poziom jakości

forma przeciwciała

serum

klon

polyclonal

reaktywność gatunkowa

human, rat

producent / nazwa handlowa

Chemicon®

metody

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

dry ice

informacje o genach

human ... CALB2(794)

Opis ogólny

Calretinin is a 29 kDa calcium binding protein that is expressed in central and peripheral nervous system and in many normal and pathological tissues. Calretinin is also considered an important diagnostic aid in the differential diagnosis of cystic and solid ameloblastic tumors. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Calretinin is generally expressed with two other calcium binding proteins Calbindin D-29 and parvalbumin in CNS and other peripheral tissues. Calretinin antibodies have been used to detect a number of carcinomas by clinicopathological, immunohistochemical techniques including, epithelial malignant mesotheliomas, Ovarian yolk sac tumor, Adenomatoid tumors of the uterus etc.

Specyficzność

Reactivity with other species has not been determined
Specific for calretinin. Recognizes both the calcium-bound and calcium-unbound conformations of calretinin by western blots.

Immunogen

Recombinant rat calretinin.

Zastosowanie

Research Category
Neuroscience
Research Sub Category
Signaling Neuroscience
This Anti-Calretinin Antibody is validated for use in IH, IH(P), WB for the detection of Calretinin.
Western blot:
1:1,000-1:2,000 of a previous lot was used.

Immunohistochemistry:
1:1,000-1:10,000
Antibody works in paraffin embedded tissues.

Optimal working dilutions must be determined by the end user.

Jakość

Immunohistochemistry(paraffin) Analysis:
Calretinin (cat. # AB5054) staining on Human Cerebellum. Tissue pretreated with Citrate, pH 6.0 plus. Antibody was diluted to 1:200 (5.0 µg/mL), using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as a nuclear and cytoplasmic punctate staining on neurons (brown).
Optimal Staining With Citrate Buffer Epitope Retrieval: Human Cerebellum.

Powiązanie

Replaces: AB149

Postać fizyczna

Rabbit polyclonal serum contains no preservative.
Unpurified

Przechowywanie i stabilność

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Komentarz do analizy

Control
Rat brain cortex, Mesothelioma tissue, Human cerebellum.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Ta strona może zawierać tekst przetłumaczony maszynowo.

Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Repeated phencyclidine in monkeys results in loss of parvalbumin-containing axo-axonic projections in the prefrontal cortex.
Bret A Morrow, John D Elsworth, Robert H Roth
Psychopharmacology (Psychopharmacologia) null
Allison Walter et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 24(9), 3360-3369 (2010-05-06)
This study evaluated the in vivo antitumor activity of grape-derived polyphenols. BALB/c mice were subcutaneously implanted with C26 colon carcinoma cells, and 2 d later they received either solvent or red wine polyphenols (RWPs) (100 mg/kg/d, human equivalent dose approximately
TRPV1 receptors modulate retinal development.
Mauro Leonelli,Daniel O Martins,Luiz R G Britto
International Journal of Developmental Neuroscience null
Nitric Oxide Synthase and Calcium-binding Protein-containing Neurons in the Hamster Visual Cortex
Lee, J.-E. et al.
Molecules and Cells, 18, 30-39 (2004)
Calcium-binding Protein Calretinin Immunoreactivity in the Dog Superior Colliculus.
Lee, Jea-Young, et al.
Acta Histochemica Et Cytochemica, 39, 125-138 (2006)

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej