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Tuner(DE3)pLysS Competent Cells - Novagen

Escherichia coli, rod shaped

• Synonim(y): Tuner Competent cells
• Kod UNSPSC: 41106202
• NACRES: NA.81

Właściwości

• product name: Tuner(DE3)pLysS Competent Cells - Novagen, Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture. T7 lysozyme expression suppresses basal T7 expression.
• pochodzenie biologiczne: Escherichia coli
• Poziom jakości: 100
• producent / nazwa handlowa: Novagen^®
• warunki przechowywania: OK to freeze; avoid repeated freeze/thaw cycles
• tryb wzrostu: adherent or suspension
• morfologia: rod shaped
• metody: microbiological culture: suitable
• transformacja komórek: transformation efficiency: >2×10⁶ cfu/μg
• Warunki transportu: dry ice
• temp. przechowywania: −70°C

Opis

Opis ogólny

Genotype: F^–ompT hsdS_B (r_B^– m_B^–) gal dcm lacY1(DE3) pLysS (Cam^R)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Tuner strains are lacZY deletion mutants of BL21 and enable adjustable levels of protein expression throughout all cells in a culture. The lac permease (lacY) mutation allows uniform entry of IPTG into all cells in the population, which produces a concentration-dependent, homogeneous level of induction. By adjusting the concentration of IPTG, expression can be regulated from very low levels up to the robust, fully induced levels commonly associated with pET vectors. Lower-level expression may enhance the solubility and activity of difficult target proteins.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.

Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture. T7 lysozyme expression suppresses basal T7 expression.

Komponenty

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlTuner(DE3)pLysS Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

Ostrzeżenie

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Informacje prawne

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 2