203118

Biotyna-NHS, rozpuszczalna w wodzie

• Synonim(y): Sulfobiotyna-NHS, sulfobursztynoimidobiotyna
• Wzór empiryczny (zapis Hilla): C₁₄H₁₈N₃O₈S₂ · Na
• Masa cząsteczkowa: 443.43
• NACRES: NA.41
• UNSPSC Code: 41116133
• Form: solid

Właściwości

• biological source: (Synthetic chemical)
• Quality Segment: 100
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated
• color: off-white
• solubility: DMSO: 10 mg/mL, distilled water: 4 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C

Opis

General description

Reacts with primary amines to biotinylate proteins under mild conditions. Since it is water-soluble, this compound can be used to biotinylate cell-surface proteins and proteins that are sensitive to organic solvents.

Water-soluble form of biotin-NHS. Water solubility is extremely convenient, eliminating the need to dissolve the reagent in an organic solvent such as dimethylformamide (DMF) and dimethylsulfoxide (DMSO) prior to use.

Preparation Note

Unstable in aqueous solution; reconstitute just prior to use. Following reconstitution in DMSO, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Analysis Note

homogeneous single spot by TLC

Other Notes

Lisanti, M.P., et al. 1989. J. Cell Biol.109, 2145.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable