The Instructions For Use for NeoClear provides a procedure for use in preparing paraffin embedded sections. Only the paraffin is heated to 60°C. All other steps are performed at room temperature. There is no available information for using NeoClear at 40°C. The Instructions for Use for NeoClear are found on the website's product detail page.
If using for deparaffinization NeoClear is similar in use to Xylene. The user may however need to slightly extend the times for each NeoClear station.
The Instructions for Use state that NeoClear is more sensitive to water. This means it may be necessary to change out the various stations of NeoClear on your staining, compared to changing out Xylene on the staining row.
1.09843
Neo-Clear™ Xylene Substitute
(xylene substitute) for microscopy
Synonim(y):
Neo-Clear™
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Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
41116113
EC Index Number:
265-067-2
NACRES:
NA.21
MDL number:
Vapor pressure:
6 hPa ( 20 °C)
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Pozwól nam pomócvapor pressure
6 hPa ( 20 °C)
Quality Segment
form
liquid
autoignition temp.
240 °C
potency
>5000 mg/kg LD50, oral (Rat), >5000 mg/kg LD50, skin (Rabbit)
expl. lim.
0.6 % (v/v)
IVD
for in vitro diagnostic use
greener alternative product characteristics
Safer Solvents and Auxiliaries
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sustainability
Greener Alternative Product
color
clear
refractive index
n20/D 1.418
kinematic viscosity
1.3 cSt(20 °C)
mp
-20 °C
transition temp
flash point 43 °C
density
0.78-0.795 g/cm3 at 20 °C
application(s)
diagnostic assay manufacturing
hematology
histology
greener alternative category
storage temp.
15-25°C
General description
Neo-Clear™ (xylene substitute) - for microscopy, is an aliphatic hydrocarbon mixture that can substitute the same applications as xylene in the histo-medical lab - histoprocessing, deparaffination and clearing after dehydration in staining process. It is not necessary to change methods or incubation times when using Neo-Clear™ instead of xylene. As the evaporation rate of Neo-Clear™ is lower then xylene, it has less odor and the disturbing smell in the lab is reduced significantly. Neo-Clear™ is more sensitive against water in solvents (turbidity can occur), thus, it is essential to avoid technical qualities of solvents. It is an IVD registered product and CE certified, thus can be used for clinical diagnostic purposes. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Legal Information
NEO-CLEAR is a trademark of Merck KGaA, Darmstadt, Germany
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Ta pozycja | |||
|---|---|---|---|
| Quality Level 400 | Quality Level - | Quality Level 400 | Quality Level 100 |
| application(s) diagnostic assay manufacturing | application(s) hematology | application(s) diagnostic assay manufacturing | application(s) - |
| form liquid | form solution | form - | form liquid |
| mp -20 °C | mp - | mp - | mp -26--23 °C (lit.) |
| expl. lim. 0.6 % (v/v) | expl. lim. - | expl. lim. - | expl. lim. 7 % |
| vapor pressure 6 hPa ( 20 °C) | vapor pressure - | vapor pressure - | vapor pressure <0.1 atm ( 21.1 °C), 16 mmHg ( 37.7 °C), 7 mmHg ( 20 °C) |
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 4 - Asp. Tox. 1 - Flam. Liq. 3
supp_hazards
Klasa składowania
3 - Flammable liquids
wgk
WGK 1
flash_point_f
110.3 °F
flash_point_c
43.5 °C
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Are there any experiences with adipose/fat tissue and NeoClear? How long should be the incubation times on 40°C?
1 answer-
Helpful?
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Could this melt the polycarbonate membranes just like in the case of xylene?
1 answer-
The product's chemical composition is "hydrocarbons, C10-C12, Isoalkanes at 100% or less. It is not known whether Neo-Clear is compatible with Polycarbonate.
Helpful?
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Is it possible to use NeoClear with paraffin tissue that was initially processed using Xylene?
1 answer-
After embedding the slides in paraffin, Neoclear or Xylene can be used to clear the slides and remove the paraffin before staining. If NeoClear is chosen, NeoMount should serve as the permanent mounting media. It is generally not recommended to use NeoClear and a xylene-based permanent mounting media, as the standard practice in histology is to use a clearing agent that matches the permanent mounting media. If an aqueous mounting media is utilized, the slides are stained, washed in water, and then coverslipped with the aqueous mounting media. In the case of aqueous mounting media, the slides do not undergo NeoClear or Xylene treatment.
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Is the solvent Neo Clear 1.09843.5000 compatible with a xylene-based mounting medium, or there is any recommendation of any other mounting medium?
1 answer-
Neo Clear is best used with Neo Mount, while Xylene should be avoided.
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