05-778
Anti-Rho (-A Antibody, -B, -C), clone 55
clone 55, Upstate®, from mouse
Synonim(y):
Aplysia ras-related homolog 12, oncogene RHO H12, ras homolog gene family, member A, small GTP binding protein RhoA
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
55, monoclonal
reaktywność gatunkowa
human, mouse, rat
producent / nazwa handlowa
Upstate®
metody
immunocytochemistry: suitable
western blot: suitable
izotyp
IgG1
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
wet ice
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... RHOA(387)
Opis ogólny
Rho proteins belong to the Ras super family of low molecular weight GTPases, which includes RAS, RHO, RAB, RAN, RAD and ARF. These small GTP binding proteins function as molecular switches that regulate a multitude of biological processes including cell proliferation, apoptosis, differentiation, cytoskeletal reorganization, and membrane trafficking.
Specyficzność
Recognizes Rho (-A, -B, -C), Mr 22 kDa.
Immunogen
Recombinant human RhoA containing amino acids 1-155. Clone 55.
Zastosowanie
Detect Rho (-A, -B, -C) using this Anti-Rho (-A Antibody, -B, -C), clone 55 validated for use in IC & WB.
Immunocytochemistry:
This antibody has been reported by an independent laboratory to detect Rho in cells via immunofluorescence.
This antibody has been reported by an independent laboratory to detect Rho in cells via immunofluorescence.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Jakość
Routinely evaluated by western blot on RIPA lysates from Jurkat or HL-60 cells.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected Rho in RIPA lysates from HL-60 cells. A previous lot detected Rho in RIPA lysates from Jurkat cells.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected Rho in RIPA lysates from HL-60 cells. A previous lot detected Rho in RIPA lysates from Jurkat cells.
Opis wartości docelowych
22 kDa
Postać fizyczna
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1 in buffer containing BSA containing 0.09% sodium azide and 50% glycerol.
Przechowywanie i stabilność
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.
Komentarz do analizy
Control
HL-60 cell lysate.
HL-60 cell lysate.
Inne uwagi
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informacje prawne
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Marina Sycheva et al.
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We have previously shown that the expression of pro-nerve growth factor (proNGF) was significantly increased, nerve growth factor (NGF) level was decreased, and the expression of p75NTR was enhanced in Alzheimer's disease (AD) hippocampal samples. NGF regulates cell survival and
Atorvastatin inhibits ABCA1 expression and cholesterol efflux in THP-1 macrophages by an LXR-dependent pathway.
Guosong Qiu,John S Hill
Journal of Cardiovascular Pharmacology null
Peroxynitrite mediates diabetes-induced endothelial dysfunction: possible role of Rho kinase activation.
El-Remessy, Azza B, et al.
Experimental Diabetes Research, 2010, 247861-247861 (2010)
Regulation of cell function by Rho GTPases.
DerMardirossian, C and Bokoch, G M
Drug News & Perspectives, 14, 389-395 (2001)
Myrto Raftopoulou et al.
Developmental biology, 265(1), 23-32 (2003-12-31)
Rho GTPases control signal transduction pathways that link cell surface receptors to a variety of intracellular responses. They are best known as regulators of the actin cytoskeleton, but in addition they control cell polarity, gene expression, microtubule dynamics and vesicular
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