04-825

Anti-PDGFRβ Antibody, clone 4A56, rabbit monoclonal

culture supernatant, clone 4A56, Upstate^®

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 4A56, monoclonal
• Application: IP, WB
• Citations: 2

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: 4A56, monoclonal
• species reactivity: mouse, human
• manufacturer/tradename: Upstate^®
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_002608
• UniProt accession no.: P09619
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... PDGFRB(5159)

Description

General description

190kDa

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 710-718 of human PDGF Receptor β. An N-terminus cysteine was added to facilitate conjugation. This sequence corresponds to amino acids 709-717 of mouse PDGF Receptor β.

Application

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

This Anti-PDGFRβ Antibody, clone 4A56 is validated for use in IP, WB for the detection of PDGFRβ.

Westerm Blot Analysis:
A 1:2,000 dilution of this lot detected PDGFRβ in RIPA lysates from mouse NIH/3T3, 3T3/A31, and human HFF cells.

Immunoprecipitation: 4 μg of a previous lot immunoprecipitated PDGFRβ from 500 μg of 3T3/A31 RIPA lysate.

Biochem/physiol Actions

PDGFRβ, does not cross-react with EGF receptor

Physical form

Cultured supernantant in 0.05% sodium azide

Preparation Note

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.

Analysis Note

Control
RIPA lysates from mouse NIH/3T3, 3T3/A31, or human HFF cells

routinely evaluated by immunoblot on RIPA lysates from mouse NIH/3T3, 3T3/A31, or human HFF cells

Other Notes

Replaces: 05-825

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable