900463
Gold nanoparticles
40 nm, maleimide functionalized, conjugation kit
Synonim(y):
Au nanoparticles
About This Item
Polecane produkty
Postać
nanoparticles
opakowanie
pkg of 1 ea
warunki przechowywania
protect from light
wielkość cząstki
40 nm
λmaks.
530 nm
grupa funkcyjna
maleimide
temp. przechowywania
−20°C
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Opis ogólny
•3 vials of 40nm Malemide-Activated Gold Nanoparticles (lyophilized)
•Protein Resuspension Buffer - 1.5ml
•Reaction Buffer - 1.5ml
•Quencher Solution - 1.5ml
•Reaction protocol
The gold nanoparticles in this kit are supplied as a lyophilized (freeze-dried) mixture. The conjugation reaction is initiated by simply reconstituting the freeze-dried nanoparticles with the protein of interest, which attaches to the nanoparticle surface via cysteine residues.
The gold conjugation procedure has a quick hands-on time of around 10 minutes, and the conjugate is fully ready for use within 3 hr. Researchers only need to pipette the biomolecule into a vial containing the nanoparticles, quench the reaction, and perform product washing through microcentrifugation.
The provided protocol is optimized for highly efficient covalent conjugation, resulting in stable gold nanoparticle conjugates. Optional protocol steps involve additional materials, such as 10% aqueous BSA (126615) and Tris saline resuspension buffer (PPB002), which are sold separately.
Zastosowanie
Different sizes of gold nanoparticles perform better in specific applications. For lateral flow and flow cytometry, we recommend using gold nanoparticles with diameters between 20 nm and 100 nm. For immunoblotting or immuno-electron microscopy, the optimal range is 5 nm to 40 nm. For bioassays utilizing dynamic light scattering, gold nanoparticles with diameters ranging from 60 nm to 100 nm are suggested.
Cechy i korzyści
•Fast and convenient conjugation reaction with no need for pre-activation.
•A spacer between the gold nanoparticle surface and the conjugated ligand minimizes the impact on the tertiary protein structure. This reduces the likelihood of poor performing conjugates, a common issue encountered with conjugates prepared by passive adsorption.
•Gold surface coating is precisely engineered to minimize non-specific protein binding in biological assays.
Informacje prawne
Kod klasy składowania
10 - Combustible liquids
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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Produkty
CRISPR/Cas9 delivery via nonviral nanoparticles shows promising advancements for gene editing in disease treatment.
Drs. Fernandes and Baptista discuss gold nanoparticles synthesis and diagnostics regulations, focusing on lateral flow assays.
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
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