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GC Analysis of cis/trans FAME Column Performance Mix on SP®-2560 per AOAC Method 2012.13

GC Analysis of cis/trans FAME Column Performance Mix on SP®-2560 per AOAC Method 2012.13 suitable for GC

Materials

analytical column

Product No.
Description
Pricing

24056

SP®-2560 Capillary GC Column

L × I.D. 100 m × 0.25 mm, df 0.20 μm

standard

Product No.
Description
Pricing

40495-U

cis/trans F.A.M.E Column Performance Mix

2.5 mg/mL in dichloromethane, ampule of 1 mL

CONDITIONS

column

SP-2560, 100 m x 0.25 mm I.D., 0.20 μm (24056)

oven

60 °C (1 min), 15 °C/min to 165 °C (1 min), 2 °C/min to 225 °C (20 min)

inj. temp.

250 °C

detector

FID, 250 °C

carrier gas

helium, 0.8 mL/min

injection

1 μL, 10:1 split

liner

4 mm I.D., split/splitless type, wool packed single taper FocusLiner™ design

sample

cis/trans FAME Column Performance Mix (40495-U)

Description

Analysis Note

Prior to analysis of samples, AOAC Method 2012.13 requires that system resolution be verified to ensure sufficient separation of the C18:1 cis/trans isomers. This is done by injecting a cis/trans FAME column performance mix (40495-U), a qualitative mix that contains a mixture of C18:1 trans and C18:1 cis isomers. Resolution is calculated between two groups of FAME isomers. Group 1 = C18:1Δ13t and C18:1Δ14t, whereas group 2 = C18:1Δ9c (oleic acid) and C18:1Δ10c. A resolution value of >1.0 is considered acceptable. As shown, the resolution value calculated using the SP-2560 column is R=1.12.

Legal Information

FocusLiner is a trademark of SGE Analytical Science Pty Ltd.
SP is a registered trademark of Sigma-Aldrich Co. LLC

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