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  • Preliminary isolation of urinary placental estriol before gas or liquid chromatography.

Preliminary isolation of urinary placental estriol before gas or liquid chromatography.

Clinical chemistry (1983-12-01)
F Andreolini, A Di Corcia, A Laganà, R Samperi, G Raponi
ABSTRACT

We describe a rapid, simple assay for placental estriol in urine, involving a small sample volume (250 microL) and, correspondingly, a small amount of enzyme reagent. After hydrolysis, estriol is adsorbed from urine onto graphitized carbon black (Carbopack B). After some washing steps, estriol is desorbed with a small volume of a suitable mobile phase. This single-step purification technique is rapid (about 15 min), with minimal sample manipulation. Analytical recoveries for estriol-supplemented urine ranged from 95.9 to 101.0%. Ten replicate analyses of urines containing typical concentrations of estriol gave CVs of 3.3, 2.6, and 2.4% for low, medium, and high concentrations, respectively. The specificity of our extraction technique was good, as assessed by treating 35 urine samples and quantifying estriol by gas chromatography with packed and capillary columns and by liquid chromatography with ultraviolet and fluorometric detection.

MATERIALS
Product Number
Brand
Product Description

Supelco
Carbopack B, stainless steel TD tube, O.D. × L 1/4 × 3 1/2, unconditioned, pkg of 10 ea
Supelco
Carbopack B, glass TD tube, fritted, O.D. × L 1/4 in. × 3 1/2 in., unconditioned, pkg of 10 ea, matrix Carbopack
Supelco
Carbopack Adsorbent, matrix Carbopack C, 60-80 mesh, bottle of 10 g
Supelco
Carbopack Adsorbent, matrix Carbopack X, 40-60 mesh, bottle of 10 g
Supelco
Carbopack Adsorbent, matrix Carbopack X, 120-400 mesh, bottle of 50 g
Supelco
Carbopack Adsorbent, matrix Carbopack C, 80-100 mesh, bottle of 10 g
Supelco
Carbopack B, O.D. × L 1/4 in. × 3 1/2 in., glass TD tube, fritted, Sealed with Brass Endcaps, preconditioned, pkg of 10 ea
Supelco
Carbopack B, glass TD tube, fritted, O.D. × L 1/4 in. × 3 1/2 in., preconditioned, pkg of 10 ea
Supelco
Carbopack Adsorbent, matrix Carbopack X, 60-80 mesh, bottle of 10 g
Supelco
FLM Carbopack X Deactivated Stainless Steel TD Tube, preconditioned, pkg of 10 tubes, O.D. × L 1/4 in. (6.35 mm) × 3 1/2 in. (89 mm)
Supelco
Carbopack Adsorbent, matrix Carbopack B, 60-80 mesh, bottle of 10 g
Sigma-Aldrich
Estriol, ≥97%
Sigma-Aldrich
Estriol, meets USP testing specifications
Supelco
Estriol solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
Supelco
Carbopack X, glass TD tube, fritted, O.D. × L 1/4 in. × 3 1/2 in., preconditioned, pkg of 10 ea