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  • A rapid TLC autographic method for the detection of glucosidase inhibitors.

A rapid TLC autographic method for the detection of glucosidase inhibitors.

Phytochemical analysis : PCA (2007-05-16)
Mario O Salazar, Ricardo L E Furlan
ABSTRACT

A new bioautographic assay suitable for the localisation of beta-glucosidase inhibitors present in a complex matrix is described. Enzyme activity was detected using esculin as the substrate to produce esculetin, which reacts with ferric ion to form a brown complex.