B8776
Botulinum Toxin A from Clostridium botulinum
lyophilized powder
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About This Item
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form
lyophilized powder
shipped in
dry ice
storage temp.
−20°C
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Biochem/physiol Actions
Inhibits release of catecholamines from adrenal medulla, acetylcholine at neuromuscular junctions.
Neurotoxin that inhibits release of catecholamines from adrenal medulla; blocks release of acetylcholine at neuromuscular junctions.
Physical form
Lyophilized powder containing 20 mM HEPES and 1.25% lactose when reconstituted with the volume of water or buffer indicated on the Certifcate of Analysis.
Preparation Note
When toxin is used for in vitro studies, it is preincubated in a buffer containing 5 mM dithiothreitol for 30 minutes in order to reduce and thereby activate the toxin.
Reconstitution
Reconstitute each 100 μg vial with 100 μL of appropriate buffer. Upon reconstitution, the vial will contain 20 mM HEPES and 1.25% lactose. To insure full recovery of toxin from the vial, include 1 mg/mL BSA in the reconstitution buffer.
Other Notes
Handle the toxin gently. Do not vortex. Use sufficient volume of buffer to wash down the walls and stopper. Read all handling information found on SDS prior to reconstitution.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 1 Oral
Storage Class Code
6.1B - Non-combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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E López-Alonso et al.
Neuroscience letters, 196(1-2), 37-40 (1995-08-18)
Giant liposomes were made from a mixture of asolectin phospholipid vesicles and presynaptic plasma membranes isolated from Torpedo cholinergic nerve endings. Acetylcholine filled giant liposomes were able to release neurotransmitter upon stimulation by the Ca2+ ionophore A23187 and Ca2+. Botulinum
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