83264

HEPES solution

1 M in H₂O, liquid

• Synonym(s): HEPES solution, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• CAS Number: 7365-45-9
• MDL number: MFCD00006158
• UNSPSC Code: 12161700
• eCl@ss: 32129211
• PubChem Substance ID: 57652951
• NACRES: NA.25

Properties

• product name: HEPES buffer solution, 1 M in H₂O
• form: liquid
• concentration: 1 M in H₂O; 238 g/L HEPES in H₂O
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• pH: 5.5±0.5
• useful pH range: 6.8-8.2
• density: 1.07 g/mL at 20 °C
• application(s): diagnostic assay manufacturing
• SMILES string: OCCN1CCN(CCS(=O)(O)=O)CC1
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used:

• As a component of SAPI medium used for the culturing of S. Typhymurium inoculum
• To prepare differentiation medium for PC12 cells. It is also a component of culture medium RPMI
• As a component of dissection buffer for culturing rat cortical neurons
• Along with MgCl₂, KCl and Spermine in hypotonic buffer in mGRASP method to detect mammalian synapses
• As a component of Danieu′s aquarium water used in agarose gel preparation

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves