MABC189

Anti-PIDD Antibody, clone 1511.1

clone 15F11.1, from mouse

• Synonym(s): p53-induced protein with a death domain, Leucine-rich repeat and death domain-containing protein, PIDD
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 15F11.1, monoclonal
• Application: ICC, WB
• Citations: -

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 15F11.1, monoclonal
• species reactivity: rat, mouse, human
• technique(s): immunocytochemistry: suitable, western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_665893
• UniProt accession no.: Q9HB75
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PIDD1(55367)

Description

General description

p53-induced protein with a death domain (PIDD) is also called Leucine-rich repeat and death domain-containing protein (LRDD). PIDD is involved in apoptosis downstream of the tumor suppressor. PIDD is a component of the DNA damage/stress response pathway in which p53/TP53 is associated with apoptosis. PIDD also aids in the activation of the transcription factor NF-kappa-B, and CASP2 which regulates apoptotic pathways.

~66 kDa observed. Uniprot describes 7 different isoforms.

Immunogen

Recombinant protein corresponding to human PIDD.

Application

Anti-PIDD Antibody, clone 1511.1 is a highly specific mouse monoclonal antibody, that targets p53-induced protein with a death domain & has been tested in western blotting & ICC.

Western Blotting Analysis: 1 µg/mL from a representative lot detected PIDD in 10 µg of NIH/3T3 and PC-12 cell lysate.

Immunohistochemistry Analysis: A 1:100 dilution, a 1:500 dilution, and a 1:1,000 dilution detected PIDD in HeLa, A431, and NIH/3T3 cells.

Biochem/physiol Actions

This antibody demonstrates specificity for isoform 4, but may detect isoform 1, 2 and 3. It will not detect isoforms 5, 6, and 7 based upon immunogen design.

Physical form

Format: Purified

Purified mouse IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Analysis Note

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected PIDD in 10 µg of HeLa cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable