CP26
Anti-Integrin β₁ Mouse mAb (4B7)
liquid, clone 4B7, Calbiochem®
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About This Item
UNSPSC Code:
12352203
biological source
mouse
Quality Level
antibody product type
primary antibodies
clone
4B7, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
should not react with
mouse, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
desiccated
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
General description
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the β1 integrin subunit.
Recognizes the β1 integrin subunit in A431 and Jurkat cells.
Immunogen
Human
recombinant, human β1 integrin
Application
Frozen Sections (2 µg/ml)
Immunofluorescence (2.5 µg/ml)
Immunoprecipitation (1 µg/sample)
Paraffin Sections (2 µg/ml, no pre-treatment required)
Immunoblotting (not recommended)
Neutralization Studies (see application references)
Immunofluorescence (2.5 µg/ml)
Immunoprecipitation (1 µg/sample)
Paraffin Sections (2 µg/ml, no pre-treatment required)
Immunoblotting (not recommended)
Neutralization Studies (see application references)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In 50 mM sodium phosphate buffer, 50% glycerol.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
A-431 or Jurkat cells or skin tissue
A-431 or Jurkat cells or skin tissue
Other Notes
Akiyama, S.K. 1996. Hum. Cell.9, 181.
Farnoud, M.R. et al. 1996. Int. J. Cancer67, 45.
Morimoto, C. and Tachibana, K. 1996. Hum. Cell.9, 163.
Webb, D.L. et al. 1996. J. Cell. Biochem.61, 127.
Schaller, M.D. et al. 1995. J. Cell. Biol.130, 1181.
Akiyama, S.K. et al. 1994. J. Biol. Chem.269, 15961.
Albelda, S.M. 1993. Lab. Invest.68, 4.
Evans, C.W. 1992. Cell. Biol. Int. Rep.16, 1.
Albelds, S.M. and Buck, C.A. 1990. FASEB J.4, 2868.
Farnoud, M.R. et al. 1996. Int. J. Cancer67, 45.
Morimoto, C. and Tachibana, K. 1996. Hum. Cell.9, 163.
Webb, D.L. et al. 1996. J. Cell. Biochem.61, 127.
Schaller, M.D. et al. 1995. J. Cell. Biol.130, 1181.
Akiyama, S.K. et al. 1994. J. Biol. Chem.269, 15961.
Albelda, S.M. 1993. Lab. Invest.68, 4.
Evans, C.W. 1992. Cell. Biol. Int. Rep.16, 1.
Albelds, S.M. and Buck, C.A. 1990. FASEB J.4, 2868.
Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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