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BugBuster^® HT Protein Extraction Reagent

• Synonym(s): Cell lysis reagent, Protein extraction buffer
• NACRES: NA.77
• UNSPSC Code: 41106511

Properties

• form: liquid
• Quality Segment: 100
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

BugBuster HT Protein Extraction Reagent combines BugBuster Protein Extraction Reagent and Benzonase^® Nuclease in one convenient reagent. BugBuster HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

Application

BugBuster^® HT Protein Extraction Reagent has been used:

• to lyse the Escherichia coli cell pellets following centrifugation for protein expression and immunoblotting
• for protein extraction from bacterial cell pellet
• used to lyse the E. coli cell pellets for the expression and purification of dMIC60 (mitofilin) protein

Features and Benefits

• Fast protein extraction and nucleic acid degradation
• Ideal for processing various samples of different volumes
• Increased extraction is ensured by the addition of rLysozyme solution

Legal Information

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2