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biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Rabbit polyclonal antibody was purified by ammonium sulfate precipitation followed by DEAE-cellulose ion exchange chromatography. Recognizes cationic trypsin.
Recognizes cationic trypsin in pancreas tissue. Does not cross-react with other plasma proteins.
This Anti-Trypsin Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunoelectrophoresis for the detection of Trypsin.
Immunogen
Human
human pancreatic trypsin
Application
ELISA (≥1:250)
Immunoblotting (1:2000)
Immunoelectrophoresis (see comments)
Immunoblotting (1:2000)
Immunoelectrophoresis (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In PBS, pH 7.4.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Human pancreas tissue
Human pancreas tissue
Other Notes
Does not cross-react with other plasma proteins. Monospecific as determined by immunoelectrophoresis against human pancreatic extract and against human trypsin. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Ji Hye Seo et al.
Digestion, 80(1), 40-49 (2009-05-30)
Proteinase-activated receptor-2 (PAR2), which is activated by trypsin, is known to be associated with expression of adhesion molecule integrins. We previously demonstrated that Helicobacter pylori induced the expression of integrin alpha(5) and beta(1) in human gastric epithelial cells. The present
Carlie S Sigel et al.
Cancer cytopathology, 121(8), 459-470 (2013-02-15)
Acinar cell neoplasms of the pancreas are rare but when encountered, the diagnosis is often established based on cytology specimens. Diagnostic accuracy is important because acinar cell carcinomas are aggressive yet may mimic tumors with different outcomes and management. The
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