04-855

Anti-GluR1 Antibody, clone C3T, rabbit monoclonal

culture supernatant, clone C3T, Upstate^®

• Synonym(s): Anti-GluR1, Anti-GluA1, Anti-GluR-1, Anti-GluR-A, Anti-GluR-K1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: C3T, monoclonal
• Application: IHC, IP, WB
• Citations: 63

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: C3T, monoclonal
• species reactivity: human, chimpanzee, rat, mouse
• manufacturer/tradename: Upstate^®
• technique(s): immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_000827.2
• UniProt accession no.: P42261
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... GRIA1(2890)

Description

General description

106 kDa

L glutamate is the major excitatory neurotransmitter in the central nervous system. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein coupled receptors that have been divided into 3 groups. Group I includes GRM1 and these receptors have been shown to activate phospholipase C. GRM1 expression has been reported in various regions of the brain and spinal cord. ESTs have been isolated from brain and eye libraries.

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 858-868 (..TLPRNSGAGAS..) of human GluR1 (amino acids 840-850 of mature human GluR1)

Application

Anti-GluR1 Antibody, clone C3T detects level of GluR1 & has been published & validated for use in IH, IP & WB.

Immunoprecipitation: 2 μL of a previous lot immunoprecipitated GluR1 from rat brain microsomal preparation.

Immunohistochemistry: A previous lot of this antibody has been shown to detect GluR1 in human, mouse and rat tissues.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Biochem/physiol Actions

Predicted to cross-react with Chimpanzee based pm 100% sequence homology.

Recognizes GluR1, Mr 106 kDa.

Physical form

Cultured supernantant in 0.05% sodium azide

Preparation Note

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Routinely evaluated by immunoblot on rat brain microsomal preparation (12-144)

Routinely evaluated by Western blotting of rat brain microsomal preparation.

Western Blotting Analysis (WB): A 1:5,000-1:10,000 dilution of this lot detected GluR1 in rat brain microsomal preparation (Catalog # 12-144).

Other Notes

Replaces: 05-855

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2