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E4643

Sigma-Aldrich

Heparin-Binding EGF-Like Growth Factor human

HB-EGF, recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

Synonym(s):

hHB-EGF, HB-EGF, Heparin-Binding Epidermal Growth Factor-Like Growth Factor

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About This Item

MDL number:
MFCD00212935
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

human

Quality Level

100

recombinant

expressed in baculovirus infected Sf21 cells

Assay

≥97% (SDS-PAGE and N-terminal analysis)

form

lyophilized powder

potency

≤3 ng/mL

quality

endotoxin tested

mol wt

9.5 kDa

packaging

pkg of 50 and 250 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

Q99075

storage temp.

−20°C

Gene Information

human ... HBEGF(1839) , HBEGF(1839)

Biochem/physiol Actions

Heparin-binding epidermal growth factor (HB-EGF), like BTC, is a heparin-binding EGF family member. Natural soluble HB-EGF is a 19 kDa glycoprotein of multiple forms due to heterogeneous truncations. Soluble HB-EGF is mitogenic and chemotactic for fibroblasts, keratinocytes, and smooth muscle cells but not capillary endothelial cells. Production of HB-EGF is detected in monocytes, macrophages, and vascular and aortic smooth muscle cells (SMC). HB-EGF may play a role in the pathogenesis of atherosclerosis. It may also be an effector of Jun-induced oncogenic transformation. HB-EGF binds to EGFR with high affinity and to ErbB with moderate affinity.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in the EGF-responsive BALB/3T3 cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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U Palmer et al.
The European respiratory journal, 14(2), 405-411 (1999-10-09)
Signalling through epidermal growth factor (EGF) receptor leads to several cellular responses including cell division and cell migration. Since EGF receptors are expressed on normal mesothelial cells, this study investigated whether EGF receptor ligands act as chemoattractants on these cells.
F Vinante et al.
Blood, 94(9), 3169-3177 (1999-11-11)
Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a widely expressed EGF superfamily member that induces mitogenic and/or chemotactic activities toward different cell types through binding to EGF receptors 1 or 4. Membrane-bound HB-EGF exerts growth activity and adhesion capabilities
M Lanzrein et al.
The Biochemical journal, 310 ( Pt 1), 285-289 (1995-08-15)
Preincubation of Vero cells with 1 microM phorbol 12-myristate 13-acetate (PMA) decreased the specific binding of diphtheria toxin by about 50%, whereas the toxic effect, endocytic uptake and membrane translocation were completely blocked. Toxin bound to PMA-treated cells was released
R Iwamoto et al.
The Journal of biological chemistry, 274(36), 25906-25912 (1999-08-28)
Heparin-binding epidermal growth factor-like growth factor (HB-EGF) transduces mitogenic signals through the EGF receptor (EGFR). There are two forms of HB-EGF, the membrane-anchored form (pro-HB-EGF) and the soluble form (sHB-EGF). We studied the biological activity of pro-HB-EGF by using a
Leticia Colyn et al.
Journal of experimental & clinical cancer research : CR, 41(1), 183-183 (2022-05-27)
Cholangiocarcinoma (CCA) is still a deadly tumour. Histological and molecular aspects of thioacetamide (TAA)-induced intrahepatic CCA (iCCA) in rats mimic those of human iCCA. Carcinogenic changes and therapeutic vulnerabilities in CCA may be captured by molecular investigations in bile, where
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