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Key Documents

HCA-7 Colony 24

9071509, human colon, Epithelial

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About This Item

UNSPSC Code:
41106514

product name

HCA-7 Colony 24, 09071509

biological source

human colon

growth mode

Adherent

karyotype

Near diploid

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human colorectal adenocarcinoma

Cell Line Description

Subpopulations isolated from the HCA-7 cell line.

DNA Profile

STR-PCR Data:

Amelogenin: X
CSF1PO: 11,14
D13S317: 9
D16S539: 10,11
D5S818: 8,12
D7S820: 11,12
THO1: 6,7
TPOX: 8,10
vWA: 14,16

Culture Medium

DMEM (High glucose Sigma D6546) + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:10 weekly using 0.05% trypsin/EDTA; 7.5% CO2; 37 °C. Suggested seeding density 2-4x10,000 cells/cm2.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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