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HPLC Analysis of Isocyanate Derivatives on Ascentis® ES-Cyano

HPLC Analysis of Isocyanate Derivatives on Ascentis® ES-Cyano application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Ascentis® ES-Cyano HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

CONDITIONS

column

Ascentis Cyano, 15 cm x 4.6 mm I.D., 5 μm particles (577306-U)

mobile phase

[A] 0.1% ammonium acetate in water; [B] 0.1% ammonium acetate in acetonitrile; (70:30, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 254 nm

injection

5 μL

sample

20 μg/mL in 80:12:8, water: acetonitrile: dimethylsulfoxide

Description

Analysis Note

This application demonstrates the suitability of Ascentis Cyano for the efficient separation of isocyanate derivatives.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany