Skip to Content

Identification and characterization of a novel Geobacillus thermoglucosidasius bacteriophage, GVE3.

Identification and characterization of a novel Geobacillus thermoglucosidasius bacteriophage, GVE3.

Archives of virology (2015-07-01)

Leonardo Joaquim van Zyl, Falone Sunda, Mark Paul Taylor, Don Arthur Cowan, Marla Iris Trindade

ABSTRACT

The study of extremophilic phages may reveal new phage families as well as different mechanisms of infection, propagation and lysis to those found in phages from temperate environments. We describe a novel siphovirus, GVE3, which infects the thermophile Geobacillus thermoglucosidasius. The genome size is 141,298 bp (G+C 29.6%), making it the largest Geobacillus spp-infecting phage known. GVE3 appears to be most closely related to the recently described Bacillus anthracis phage vB_BanS_Tsamsa, rather than Geobacillus-infecting phages described thus far. Tetranucleotide usage deviation analysis supports this relationship, showing that the GVE3 genome sequence correlates best with B. anthracis and Bacillus cereus genome sequences, rather than Geobacillus spp genome sequences.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Methanol-¹²C, 99.95 atom % ¹²C

Sigma-Aldrich

Phenol, ≥99%

Sigma-Aldrich

Phenol, puriss. p.a., ACS reagent, reag. Ph. Eur., 99.0-100.5%

Sigma-Aldrich

Sodium dodecyl sulfate solution, BioUltra, for molecular biology, 20% in H₂O

Sigma-Aldrich

Phenol, puriss., meets analytical specification of Ph. Eur., BP, USP, 99.5-100.5% (GC)

Sigma-Aldrich

Sodium dodecyl sulfate solution, BioUltra, for molecular biology, 10% in H₂O

Sigma-Aldrich

Isoamyl alcohol, ≥98%, FG

Sigma-Aldrich

Isoamyl alcohol, natural, ≥98%, FG

Sigma-Aldrich

Phenol, for molecular biology

Sigma-Aldrich

Phenol, BioXtra, ≥99.5% (GC)

Sigma-Aldrich

Liquified Phenol, ≥89.0%

Sigma-Aldrich

Phenol solution, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology

Sigma-Aldrich

Phenol, ACS reagent, ≥99.0%

Sigma-Aldrich

Methanol, NMR reference standard

Sigma-Aldrich

Sodium dodecyl sulfate, ACS reagent, ≥99.0%

Sigma-Aldrich

Sodium dodecyl sulfate, ≥98.0% (GC)

Sigma-Aldrich

3-Methyl-1-butanol, anhydrous, ≥99%

Sigma-Aldrich

Chloroform, anhydrous, ≥99%, contains 0.5-1.0% ethanol as stabilizer

Sigma-Aldrich

Sodium dodecyl sulfate, BioXtra, ≥99.0% (GC)

Sigma-Aldrich

Sodium dodecyl sulfate, ReagentPlus^®, ≥98.5% (GC)

Sigma-Aldrich

Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

Sigma-Aldrich

Sodium dodecyl sulfate, BioUltra, for molecular biology, ≥99.0% (GC)

Supelco

Sodium dodecyl sulfate, dust-free pellets, suitable for electrophoresis, for molecular biology, ≥99.0% (GC)

Sigma-Aldrich

Methanol, anhydrous, 99.8%