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  • Construction of a thiamin sensor from the periplasmic thiamin binding protein.

Construction of a thiamin sensor from the periplasmic thiamin binding protein.

Chemical communications (Cambridge, England) (2010-12-03)
Jeremiah W Hanes, Debashree Chatterjee, Erika V Soriano, Steven E Ealick, Tadhg P Begley
PMID21125117
ABSTRACT

This communication describes the development of a thiamin sensor based on the bacterial thiamin binding protein. A triple mutant (C48S, C50S, S62C) of TbpA was labeled on C62 with N-[2-(L-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide (MDCC). Thiamin binding to this protein reduced the coumarin fluorescence giving a thiamin sensor with low nanomolar sensitivity.