Complete determination of natural site-specific enantio-isotopomeric excesses in linoleic acid using natural abundance deuterium 2D NMR in polypeptide mesophases.

NAD 2D NMR spectroscopy using chiral mesophases made of poly-gamma-benzyl-l-glutamate and pyridine allowed us to evaluate, for the first time, the natural site-specific enantio-isotopomeric excesses at each methylene group of linoleic acid, a central, essential PUFA precursor of all conjugated triene fatty acids.