Skip to Content
Merck
  • Immunohistochemical staining of epoxy resin sections of peripheral nerve.

Immunohistochemical staining of epoxy resin sections of peripheral nerve.

Applied immunohistochemistry & molecular morphology : AIMM (2005-08-06)
Zhao Cai, Jim Manavis, Kathy Cash, Philip D Thompson, Peter C Blumbergs
PMID16082259
ABSTRACT

The authors describe a simple and cost-effective method of immunostaining semithin epoxy resin sections of peripheral nerve for light microscopy with antibodies to myelin protein zero, peripheral myelin protein 22, myelin basic protein, and neurofilament protein 200 using a combined technique of surface etching with sodium ethoxide and heat-induced antigen retrieval.