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  • Inhibition of bovine kidney low molecular mass phosphotyrosine protein phosphatase by uric acid.

Inhibition of bovine kidney low molecular mass phosphotyrosine protein phosphatase by uric acid.

Journal of enzyme inhibition and medicinal chemistry (2003-04-10)
José Mauro Granjeiro, Carmen Verissima Ferreira, Paulo Afonso Granjeiro, Cinthia Celestino Da Silva, Eulázio Mikio Taga, Pedro Luiz Onofre Volpe, Hiroshi Aoyama
ABSTRACT

Uric acid inhibited 50% of the activity of bovine kidney low molecular mass phosphotyrosine protein phosphatase at concentrations of 1.0, 0.4, 1.3, and 0.2 mM, respectively for p-nitrophenyl phosphate (p-NPP), flavine mononucleotide, beta-naphthyl phosphate and tyrosine phosphate (Tyr-P) as substrates. The mixed type inhibition of p-NPP hydrolysis was fully reversible, with Kic and Kiu values of 0.4 and 1.1 mM, respectively; the inhibition by uric acid shifted the pH optimum from 5.0 to 6.5. When Tyr-P was the substrate, competitive inhibition was observed with a Ki value of 0.05 mM. Inhibition studies by uric acid in the presence of thiol compounds, and preincubation studies in the presence of inorganic phosphate suggest that the interaction of uric acid with the enzyme occurred at the active site, but did not involve SH residues, and that the mechanism of inhibition depended on the structure of the substrates.