- Structure of the human 20S U5 snRNP.
Structure of the human 20S U5 snRNP.
Nature structural & molecular biology (2024-03-12)
Sarah Schneider, Irina Brandina, Daniel Peter, Sonal Lagad, Angelique Fraudeau, Júlia Portell-Montserrat, Jonas Tholen, Jiangfeng Zhao, Wojciech P Galej
PMID38467877
ABSTRACT
The 20S U5 small nuclear ribonucleoprotein particle (snRNP) is a 17-subunit RNA-protein complex and a precursor of the U4/U6.U5 tri-snRNP, the major building block of the precatalytic spliceosome. CD2BP2 is a hallmark protein of the 20S U5 snRNP, absent from the mature tri-snRNP. Here we report a high-resolution cryogenic electron microscopy structure of the 20S U5 snRNP, shedding light on the mutually exclusive interfaces utilized during tri-snRNP assembly and the role of the CD2BP2 in facilitating this process.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody produced in mouse, clone M2, purified immunoglobulin, buffered aqueous glycerol solution