- Stimulation of B cells by sequential addition of anti-immunoglobulin antibody and cytochalasin.
Stimulation of B cells by sequential addition of anti-immunoglobulin antibody and cytochalasin.
Journal of immunology (Baltimore, Md. : 1950) (1986-02-01)
T L Rothstein
PMID3484500
ABSTRACT
B cells are stimulated to initiate DNA synthesis by modest doses of anti-immunoglobulin antibody in combination with cytochalasin. The ability of these agents to stimulate B cells in a sequential fashion was evaluated. Anti-immunoglobulin prepared cells to respond to subsequently added cytochalasin, but cytochalasin did not prepare cells to respond to anti-immunoglobulin. Only brief exposure to anti-immunoglobulin was needed to activate B cells for cytochalasin responsiveness; once activated, B cells remained responsive to cytochalasin for hours. The ability of cytochalasin to supply a second signal to activated B cells suggests that the cytoskeleton may be involved in generating or transducing growth-promoting signals for B lymphocytes.
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