This item is a multi-component product. A CAS number cannot be applied to this type of product. The Safety Data Sheet document includes the individual CAS numbers of those components that may be potentially hazardous.
D8537
Dulbecco′s Phosphate Buffered Saline
Dulbecco’s Phosphate Buffered Saline
1 of 6 reviewers received a sample product or took part in a promotion
liquid, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
DPBS
Sign Into View Organizational & Contract Pricing
Select a Size
100 ML
500 ML
1 L
6 X 500 ML
All Photos(1)
Select a Size
Change View
100 ML
500 ML
1 L
6 X 500 ML
About This Item
Recommended Products
Product Name
Dulbecco′s Phosphate Buffered Saline, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
glucose: no (Dextro)
sodium pyruvate: no
shipped in
ambient
Looking for similar products? Visit Product Comparison Guide
General description
Dulbecco’s Phosphate Buffered Saline (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS buffer is used to irrigate, wash, and dilute mammalian cells while maintaining osmotic balance. It provides water and inorganic ions essential for normal cell metabolism. DPBS with calcium and magnesium facilitates cell binding and clumping. Dulbecco′s PBS without these ions can be used to wash and rinse suspended cells. Phosphate buffering maintains the pH in the physiological range.
Application
Dulbecco′s Phosphate Buffered Saline has been used:
also commonly purchased with this product
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Arturo Levican et al.
Applied and environmental microbiology, 79(16), 4951-4957 (2013-06-19)
The genus Arcobacter is composed of 17 species which have been isolated from various sources. Of particular interest are A. butzleri, A. cryaerophilus, and A. skirrowii, as these have been associated with human cases of diarrhea, the probable transmission routes
Elliptical optical tweezers for trapping a red blood cell aggregate
Kauppila AJ
Photonics Letters of Poland, 3(3), 95-97 (2011)
Casper Hyttel Clausen et al.
Sensors (Basel, Switzerland), 18(10) (2018-10-20)
Monitoring of bacteria concentrations is of great importance in drinking water management. Continuous real-time monitoring enables better microbiological control of the water and helps prevent contaminated water from reaching the households. We have developed a microfluidic sensor with the potential
Cameron Goertzen et al.
Biological procedures online, 20, 20-20 (2018-10-26)
Invadopodia are actin-rich, proteolytic structures that enable cancer cell to invade into the surrounding tissues. Several in vitro invasion assays have been used in the literature ranging from directional quantitative assays to complex three-dimensional (3D) analyses. One of the main
Stereological monitoring of mouse lung alveolarization from the early postnatal period to adulthood.
Agnieszka Pozarska et al.
American journal of physiology. Lung cellular and molecular physiology, 312(6), L882-L895 (2017-03-21)
Postnatal lung maturation generates a large number of small alveoli, with concomitant thinning of alveolar septal walls, generating a large gas exchange surface area but minimizing the distance traversed by the gases. This demand for a large and thin gas
Protocols
Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.
Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.
Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.
Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.
-
Bonjour, pourriez-vous m'indiquer le numéro de CAS de la solution de phosphate saline dulbecco sous forme liquide
1 answer-
Helpful?
-
-
Hi, is this product GMP-grade or are GMP-garde material is used for the production of this?
1 answer-
This product is not GMP-grade. Unfortunately. a GMP-grade version of this product is not available.
Helpful?
-
-
What is the concentration of the product in Molarity?
1 answer-
This is a multi-component formulation. A 'molarity' is not reported for this type of product. See below for the list of components:
Product D8537 contains:
Potassium chloride: 0.2 g/l
Potassium phosphate monobasic: 0.2 g/l
Sodium chloride: 8 g/l
Sodium Phosphate dibasic: 1.15 g/lPlease see the link below for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/124/464/d8537for.pdfHelpful?
-
-
What is the bottle thread type on the 1L bottle (D8537-1L)
1 answer-
The product is packaged in a square PET bottle with an HDPE cap. The cap specifications are as follows:
Thread Type 38-430
Thread Diameter 37.3 mmHelpful?
-
-
What is the formulation for Product D8537, Dulbecco's Phosphate?
1 answer-
The formulation for 1 liter is 8 g Sodium Chloride, 0.2 g Potassium Phosphate, monobasic, 1.15 g Sodium Phosphate, dibasic, and 0.2 g Potassium Chloride.
Helpful?
-
-
What is a Salt Toxicity Test?
1 answer-
The Cell Culture assay is called a Salt Toxicity Test (previously called Cell Culture Salt Testing). A more complete description is below:Cells are harvested and counted. They are resuspended in DPBS and incubated at 37°C, 5% CO2 for 1 hour. The cells are counted and %viability determined. The cells are then cultured for an additional 7 hours (total of 8 hours). The cells are counted again and the % viability determined. The viability at 1 hr has to be >95% of the original viability. The viability at 8 hrs must be >75% of the original viability.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
What is the pH of a solution of Product D8537, Dulbecco's Phosphate?
1 answer-
The pH will be in the range of 7.1-7.5, according to the specification sheet.
Helpful?
-
-
Is Product D8537, Dulbecco's Phosphate, a 1X or 10 X concentration?
1 answer-
This is a 1X concentration. The 10X concentration is listed under product D1408.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
