E6635

Ethylenediaminetetraacetic acid disodium salt dihydrate

Sigma Grade, suitable for plant cell culture, 98.5-101.5%

• Synonym(s): Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na₂, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na₂
• Empirical Formula (Hill Notation): C₁₀H₁₄N₂Na₂O₈ · 2H₂O
• CAS Number: 6381-92-6
• Molecular Weight: 372.24
• Beilstein: 3900609
• EC Number: 205-358-3
• UNSPSC Code: 12352107
• PubChem Substance ID: 24894632
• NACRES: NA.77

Properties

• grade: Sigma Grade
• Quality Level: 200
• Assay: 98.5-101.5%
• form: powder
• reaction suitability: reagent type: chelator
• technique(s): cell culture | plant: suitable
• mp: 248 °C (dec.) (lit.)
• solubility: H₂O: soluble, clear, colorless
• SMILES string: O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
• InChI: 1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2
• InChI key: OVBJJZOQPCKUOR-UHFFFAOYSA-L

Description

General description

Ethylenediaminetetraacetic acid (EDTA) is an aminopolycarboxylic acid and a hexadentate ligand. It chelates with metal ions, especially with cations to form an octahedral complex.[1] Ethylenediaminetetraacetic acid disodium salt (EDTA) is a blood anticoagulant and contributes to the pathogenesis of pseudothrombocytopenia. It chelates with calcium in the blood and inhibits clotting and is routinely used in haematological tests.[2] It enhances the antibacterial activity of lysozyme.[3] EDTA used in chelation therapy chelates with calcium and favors dilation of artery, solubilisation of atheromatous plaques in atherosclerotic vascular disease.[4] EDTA chelation therapy may also protect from oxidative damage during blood and lipid peroxidation in liver fibrosis. [5]

Application

Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Ethylenediaminetetraacetic acid disodium salt dihydrate has been in seed germination trials of plant species[6] and in protein extraction from Moss, Physcomitrella paten.[7] It has also been used in lysis and vacuole buffer for the isolation of vacuoles from Petunia petals.[8]

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Warning
• Hazard Statements: H332,H373
• Precautionary Statements: P260 - P271 - P304 + P340 + P312 - P314 - P501
• Hazard Classifications: Acute Tox. 4 Inhalation - STOT RE 2 Inhalation
• Target Organs: Respiratory Tract
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves