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  • [Content determination of two isomers containd in Garcinia hanburyi by HPLC].

[Content determination of two isomers containd in Garcinia hanburyi by HPLC].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (2013-02-13)
Jun-Yan Zhang, Guang-Ping Xia, Na-Xia Zhao, Yun-Ping Chang, Ying-Mei Han
ABSTRACT

To establish a method for determing the content of two isomers containd in Garcinia hanburyi by HPLC. Chromatographic column of SunFire (Waters) C8 (2.1 mm x 150 mm, 3.5 microm) was adopted, with acetonitrile-methanol-0.3% trifluoroacetic acid (36: 37:27) as the mobile phase. The detection wavelength was 360 nm,the flow rate was 0.3 mL x min(-1), and the column temperature was 28 degrees C. The linear regression equation of r-gambogic acid was Y = 2.87 x 10(6) X - 2.24 x 10(5), r = 0.999 9. The linear regression equation of S-gambogic acid was Y = 3.31 x 10(6) X - 1.44 x 10(5), r = 0.999 9. The average recoveries were 100.0% and 100.9%, with RSD being 2.1% and 2.5% (n = 6), respectivley. The average contents of two gambogic acid in G. hanburyi were 30.06% and 21.45%, respectively. The method was so convenient and stable that it can be used for identification and content determination of two isomers containd in G. hanburyi.

MATERIALS
Product Number
Brand
Product Description

Supelco
Discovery® C8 Supelguard Guard Cartridge, 5 μm particle size, L × I.D. 2 cm × 2.1 mm
Supelco
Discovery® C8 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2.1 mm
Supelco
Discovery® C8 HPLC Column, 5 μm particle size, L × I.D. 12.5 cm × 4.6 mm
Supelco
Discovery® C8 HPLC Column, 5 μm particle size, L × I.D. 12.5 cm × 4 mm
Supelco
Discovery® C8 Supelguard Guard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm
Supelco
Discovery® C8 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
Supelco
Discovery® C8 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Supelco
Discovery® C8 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 3 mm
Supelco
Discovery® C8 HPLC Column, 5 μm particle size, L × I.D. 5 cm × 2.1 mm
Supelco
Discovery® C8 HPLC Column, 5 μm particle size, L × I.D. 5 cm × 4.6 mm
Supelco
Discovery® C8 Supelguard Guard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm