66828-U

BIOshell A400 Protein C4 (3.4 μm) HPLC Columns

L × I.D. 10 cm × 4.6 mm, HPLC Column

• Synonym(s): Superficially Porous Wide Pore C4 UHPLC Column
• UNSPSC Code: 41115700
• NACRES: SB.52

Properties

• product name: BIOshell A400 Protein C4, 3.4 μm HPLC Column, 3.4 μm particle size, L × I.D. 10 cm × 4.6 mm
• material: stainless steel hardware
• Quality Level: 100
• Agency: suitable for USP L26
• description: Shell thickness (0.2 μm); Solid Core (3.0 μm)
• product line: BIOshell
• feature: endcapped
• manufacturer/tradename: BIOshell
• packaging: 1 ea of
• extent of labeling: 0.4% carbon loading
• parameter: 600 bar max. pressure; 90 °C max. temp.
• technique(s): HPLC: suitable; LC/MS: suitable; UHPLC-MS: suitable; UHPLC: suitable
• L × I.D.: 10 cm × 4.6 mm
• surface area: 15 m²/g
• surface coverage: 4.2 μmol/m²
• matrix: spherical silica particle platform; superficially porous particle
• matrix active group: C4 (butyl) phase
• particle size: 3.4 μm
• pore size: 400 Å
• operating pH range: 2-9
• separation technique: reversed phase

Description

General description

BIOshell 400 Å Protein C4 columns contain 3.4 μm advanced Fused-Core particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins. These columns provide excellent separations on very hydrophobic proteins such as those found in the cell membrane. The C4 ligand combined with an exhaustively endcapped surface ensures optimum protein recovery of larger or very hydrophobic biomolecules. The 3.4 μm Fused-Core particles allow a shorter analysis time yet similar back pressure to fully porous 1.7 μm, 300 Å fully porous C4 particles. These columns can be operated long term at pressures up to 9000 psi (600 bar), temperatures up to 90 °C, and within the pH range of 2 to 9.

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