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Key Documents

AC-0368

Sigma-Aldrich

p62 (Sequestosome-1) (EP396) Rabbit Monoclonal Primary Antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

Ig fraction of antiserum

clone

monoclonal

description

For In Vitro Diagnostic Use in Select Regions (See Chart)

form

buffered aqueous solution

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

isotype

IgG

shipped in

wet ice

storage temp.

2-8°C

visualization

(nuclear,cytoplasm)

General description

P62, also known as Sequestosome-1, is a ubiquitin-binding adapter protein involved in the delivery of ubiquitin-bound protein and organelles to the autophagosome for lysosomal degradation during autophagy. Mutations in p62 have been linked to Paget′s disease and neurodegenerative disorders. P62 is typically degraded during the autophagy process, but can accumulate and/or overexpressed in autophagy-deficient cells. Presence of p62 cytosolic aggregate is used as a marker for autophagy deficiency. Defective autophagy increase oxidative stress and may play a role in tumorigenesis. P62 immunohistochemistry (IHC) diagnostic utility was established for identifying hepatocellular carcinoma (HCC). P62 demonstrates superior sensitivity for HCC compared against glypican-3. Positive p62 staining was found in 100% of HCC but were negative in all non-tumor areas and cirrhotic nodules. Furthermore, a panel consisting of p62, aminoacylase 1, and glypican-3 provides high sensitivity (93.8%) and specificity (95.2%) in the differential diagnosis between well differentiated HCC and high grade dysplastic nodules. P62 expression can also aid in diagnosing drug-induced autophagic vacuolar myopathies. An optimal threshold of at least 11.5% positive fibers provided 100% sensitivity and 100% specificity for autophagic myopathy. While current diagnostic criteria require identification of autophagic vacuoles by electron microscopy, diagnosis with p62 IHC could expedite the process and reduce costliness and large sampling variance associated with electron microscopy.

Quality


IVD

IVD

IVD

RUO

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and &lt0.1% Sodium Azide

Other Notes

For a copy of the IFU and CofA contact IVDorder@ABCAM.com

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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