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Roche

RNA, MS2

from bacteriophage MS2

Synonym(s):

RNA, ribonucleic acid

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About This Item

UNSPSC Code:
41106311

biological source

bacteriophage MS2

Quality Level

form

solution

mol wt

~1200 kDa

packaging

pkg of 500 μL (10 A260 units)

manufacturer/tradename

Roche

technique(s)

RNA extraction: suitable

color

colorless

solubility

water: miscible

storage temp.

−20°C (−15°C to −25°C)

Related Categories

General description

RNA, from bacteriophage MS2 is composed of 3569 nucleotides. MS2 bacteriophage acts as a model system in studying molecular biology processes, such as viral RNA replication, process of translation and physiology of infected cells. MS2 RNA codes for three viral polypeptides namely, protein A, coat protein and RNA replicase complex. Protein A and coat protein make up the structure of MS2 virus.

Application

RNA, MS2 has been used:
  • as a standard to assess RNA quantification methods
  • in RNA extraction
  • in single-tube, fluorescent PERT assay (STF-PERT) for accurate quantitation of different retrovirus types
  • for studies which use natural RNA in a in vivo and in vitro protein-synthesizing system, initiation, elongation, and termination of polypeptide synthesis.
  • for structural and functional studies.
  • to stabilize RNA during cDNA synthesis
  • to improve RNA yield during RNA extraction or isolation

Quality

Typical analysis: Free of protein and host nucleic acids according to current Quality Control procedures.

Sequence

Chain Length 3,569 nucleotides

Preparation Note

Working solution: Storage buffer: 10 mM Tris-HCl, 1 mM EDTA, pH 7.0.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

No data available

Flash Point(C)

No data available


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Dennis M Timmerman et al.
Cancers, 13(17) (2021-09-11)
MicroRNAs (miRNAs) are short, non-coding RNAs involved in translation regulation. Dysregulation has been identified in cancer cells. miRNAs can be secreted and detectable in body fluids; therefore, they are potential liquid-biopsy biomarkers. The miR-371a-3 cluster members are an example, monitoring
Yun Kun Ma et al.
Journal of virological methods, 157(2), 133-140 (2009-02-03)
PCR-based reverse transcriptase (RT) assays are highly sensitive for broad detection of retroviruses. These assays are currently used for demonstrating the absence of retroviral contamination in vaccines and can also be applied to clinical and laboratory research to investigate low-virus
Małgorzata Guz et al.
BioMed research international, 2020, 4250329-4250329 (2020-08-11)
Inflammatory bowel disease (IBD) comprises ulcerative colitis (UC) and Crohn disease (CD). Similar symptoms, but different treatment procedures for both diseases require precise diagnosis. MicroRNAs (miRNAs) are major posttranscriptional players that regulate the expression of genes during the inflammation and
Kirstine Kirkegaard et al.
Journal of assisted reproduction and genetics, 37(9), 2199-2209 (2020-07-19)
miRNAs have been suggested as biomarkers of embryo viability; however, findings from preliminary studies are divergent. Furthermore, the presence of other types of small RNA molecules remains to be investigated. The purpose of this study was to perform a comprehensive
Michael Eikmans et al.
International journal of molecular sciences, 21(11) (2020-06-12)
About 10-15% of couples who want to conceive suffer from subfertility, while in 30% of these cases, a male factor plays a role. Levels of particular microRNAs in seminal plasma, including those involved in spermatogenesis, may serve as an indicative

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