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Key Documents

MAB2247-I

Sigma-Aldrich

Anti-Integrin β1 Antibody, clone 12G10

clone 12G10, from mouse

Synonym(s):

Integrin beta-1, CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

12G10, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable
immunofluorescence: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ITGB1(3688)

General description

Integrin beta-1 (UniProt P05556; also known as CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12, VLA-BETA, VLAB) gene (Gene ID 3688) in human. Integrin beta-1 is a 130 kDa transmembrane glycoprotein that interact with various integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression.

Immunogen

Purified human β1 Integrin preparation from HT1080 fibrosarcoma cell extract

Application

Research Category
Cell Structure
Research Sub Category
ECM Proteins
This Anti-Integrin β1 Antibody, clone 12G10 is validated for use in Western Blotting, Flow Cytometry, Immunofluorescence for the detection of Integrin β1.
Western Blot Analysis: 0.5 µg/mL from a representative lot detected Integrin β1 in 10 µg of Integrin purified protein.

Immunofluorescence Analysis: A representative lot was used in an independent laboratory in mouse lung tissue. (Chen P., et al. (2009). PLoS ONE. 4(8):e6565.)

Quality

Evaluated by Flow Cytometry in HeLa cells.

Flow Cytometry Analysis: 1 µg of this antibody detected Integrin β1 in HeLa cells.

Target description

~192 kDa observed

Linkage

Replaces: MAB2247

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in PBS containing 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Peter Chen et al.
PloS one, 4(8), e6565-e6565 (2009-08-12)
Lung injury promotes the expression of matrix metalloproteinase-7 (MMP7, matrilysin), which is required for neutrophil recruitment and re-epithelialization. MMP7 governs the lung inflammatory response through the shedding of syndecan-1. Because inflammation and repair are related events, we evaluated the role

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