ABN1369
Anti-Substance P Receptor
from rabbit, purified by affinity chromatography
Synonym(s):
Substance-P receptor, Neurokinin-1 receptor, NK-1 receptor, NK-1R, SPR, Tachykinin receptor 1
Sign Into View Organizational & Contract Pricing
All Photos(4)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, human, mouse
species reactivity (predicted by homology)
canine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... TACR1(6869)
General description
Substance-P receptor (UniProt P25103; also known as Neurokinin-1 receptor, NK-1 receptor, NK-1R, SPR, Tachykinin receptor 1) is encoded by the TACR1 (also known as NK1R, TAC1R) gene (Gene ID 6879) in human. NK-1, NK-2 and NK-3 are receptors that mediate the biological activities of tachykinins, including substance P (SP), neurokinin A (NKA), neurokinin B (NKB), ranakinin, kassinin, neuropeptide gamma, and eledoisin, widely distributed in the central and peripheral nervous systems. SP shows the highest affinity toward NK-1R (substance-P receptor) and exerts its biological activities via NK-1R, including addiction, depression, chronic inflammation, and cancer progression. The involvement of NK-1R in acute pancreatitis through mediating neurogenic inflammation has also been reported. Upon stimulation by SP, NK-1R interacts with multiple G proteins, including G s, G q/11, G i/o, G 12, and G 13, generating second messengers and activating downstream effector molecules. After binding NK-1R, SP induces a clathrin-dependent internalization of the receptor-ligand complex, SP is then degraded and the receptor is recycled to the cell surface. NK-1R is a 7-transmembrane (32-54, 65-86, 107-128, 149-169, 195-219, 249-270, 284-308) GPCR, having its N-terminal end (a.a. 1-31) at the extracelluar side and C-terminal end (a.a. 309-407) at the cytoplasmic side. There exists a second alternatively spliced human NK-1R isoform that lacks the C-terminal cytoplasmic domain (a.a. 312-407 missing). The full-length form enhances the growth of cancer cells and stimulates the production of cytokines, which in turn up-regulate the truncated form. The truncated form mediates malignancy and is more abundant in colonic epithelial cells from patients with colitis-associated cancer. In cancer cells, the NK-1 receptor is mainly localized on the plasma membrane and in cytoplasm, and occasionally also in the nucleus.
Specificity
This rabbit polyclonal antibody targets substance P receptor C-terminal end sequence. Target region is absent in human spliced isoform 2.
Immunogen
KLH-conjugated linear peptide corresponding to the C-terminal end sequence of human substance P receptor.
Application
Detect Substance P receptor using this rabbit polyclonal Anti-Substance P Receptor Antibody, Cat. No. ABN1369, validated for use in Immunohistochemistry and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected substance P receptor in human, mouse, and rat brain tissue sections.
Quality
Evaluated by Western Blotting in rat brain tissue lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected substance P receptor in 10 µg of rat brain tissue lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected substance P receptor in 10 µg of rat brain tissue lysate.
Target description
~58 kDa observed. Target band size appears larger than the calculated molecular weights of 46.25/46.32/46.37 kDa (human/mouse/rat) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).
Other Notes
Concentration: Please refer to lot specific datasheet.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Silvia Gutierrez et al.
Cancer cell international, 18, 216-216 (2019-01-02)
Metastatic cancer to bone is well-known to produce extreme pain. It has been suggested that the magnitude of this perceived pain is associated with disease progression and poor prognosis. These data suggest a potential cross-talk between cancer cells and nociceptors
Yan-Jia Luo et al.
Nature communications, 9(1), 1576-1576 (2018-04-22)
Nucleus accumbens (NAc) is involved in behaviors that depend on heightened wakefulness, but its impact on arousal remains unclear. Here, we demonstrate that NAc dopamine D1 receptor (D1R)-expressing neurons are essential for behavioral arousal. Using in vivo fiber photometry in
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service