Skip to Content
Merck
All Photos(1)

Documents

17-285

Sigma-Aldrich

TCF Reporter Plasmid Kit

Set of transfection grade T cell factor (TCF) reporter plasmids for use in TOPFlash and FOPFlash wnt/b-catenin activity assays.

Synonym(s):

TOPFlash/FOPFlash, TOPFlash plasmid, FOPFlash plasmid

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.51

Quality Level

manufacturer/tradename

Upstate®

technique(s)

transfection: suitable

NCBI accession no.

UniProt accession no.

application(s)

genomic analysis

shipped in

dry ice

General description

FOPflash: Transfection grade T cell factor (TCF) reporter plasmid containing two full and one incomplete copy of the TCF binding site (mutated) followed by three copies in the reverse orientation, upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. This plasmid serves as a negative control to TOPflash which contains wild type TCF binding sites.

TOPflash: Transfection grade T-cell factor (TCF) reporter plasmid containing two sets (with the second set in the reverse orientation) of three copies of the TCF binding site (wild type) upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. FOPflash containing mutated TCF binding sites is also available as a negative control.

The Wnt family encodes secreted glycoproteins implicated in cell growth regulation, cell fate determination, organogenesis and oncogenesis. Inferred from genetic analysis of wingless (wg) signaling in Drosophila, in mammalian cells, Wnts signal through the Frizzled family of receptors, to Dishevelled. Dishevelled down regulates glycogen synthase kinase-3ß (GSK-3ß) activity. This results in the stabilization and accumulation of cytosolic ß-catenin, a GSK-3ß substrate, which when phosphorylated by GSK-3ß, is targeted for ubiquitin-mediated proteolysis. ß-catenin then translocates to the nucleus, where in complexes with members of the Tcf/LEF family of transcription regulators, activates transcription of Tcf-responsive genes. This Tcf-reporter plasmid, along with FOPFLASH (Cat. # 21-169) enables quantitation of Wnt/Wg signaling in cells transfected with these constructs.

Application

Set of transfection grade T cell factor (TCF) reporter plasmids for use in TOPFlash and FOPFlash wnt/b-catenin activity assays.

Components

FOPflash (mutant TCF binding sites) (Cat.# 21-169)

TOPflash (TCF Reporter Plasmid) (Cat.# 21-170)

Quality

Routinely evaluated by DNA Sequence Analysis to confirm the identity of TCF binding sites.

Physical form

FOPflash, Catalog # 21-169, Lot # 30860, 5.5 kb. 5 μg affinity purified DNA eluted and aseptically packaged in 50 μL of 10 mM Tris-HCl, pH 8.0, 1 mM EDTA. Frozen solution.

TOPflash, Catalog # 21-170, Lot # 27752, 5.5 kb. 5 μg affinity purified DNA eluted and aseptically packaged in 50 μL of 10 mM Tris-HCl, pH 8.0, 1 mM EDTA. Frozen solution.

Storage and Stability

Stable for 2 years at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Yan-Jiun Huang et al.
American journal of cancer research, 10(8), 2337-2354 (2020-09-10)
Patients with advanced-stage colon cancer often exhibit resistance against treatment and distant metastasis, both key contributors to poor prognosis. Emerging evidence indicates that cancer stem cells (CSCs), characterized by the enhanced ability to self-renew, resist therapeutics, and promote metastasis, represents
B Klucky et al.
Oncogene, 26(43), 6356-6360 (2007-04-18)
DNA tumor viruses are capable of driving quiescent cells into the cell cycle. In case of polyomaviridae, two viral proteins, the large and the small (ST) T antigens are responsible for this outcome. ST interacts with the protein phosphatase PP2A
Karthik Aripaka et al.
EBioMedicine, 45, 192-207 (2019-07-03)
Tumour necrosis factor receptor associated factor 6 (TRAF6) promotes inflammation in response to various cytokines. Aberrant Wnt3a signals promotes cancer progression through accumulation of β-Catenin. Here we investigated a potential role for TRAF6 in Wnt signaling. TRAF6 expression was silenced
F J Staal et al.
International immunology, 11(3), 317-323 (1999-04-30)
Beta-catenin is the vertebrate homolog of the Drosophila segment polarity gene Armadillo and plays roles in both cell-cell adhesion and transduction of the Wnt signaling cascade. Recently, members of the Lef/Tcf transcription factor family have been identified as protein partners
Zhongyan Zhang et al.
Journal of molecular and cellular cardiology, 46(3), 370-377 (2008-12-27)
We have demonstrated that mesenchymal stem cells overexpressing the survival gene Akt can confer paracrine protection to ischemic myocytes both in vivo and in vitro through the release of secreted frizzled related protein 2 (Sfrp2). However, the mechanisms mediating these

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service