05-928
Anti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonal
clone A3S, Upstate®, from rabbit
Synonym(s):
H3, Histone H3, H3 histone, family 3A
About This Item
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
A3S, monoclonal
species reactivity
Saccharomyces cerevisiae, mouse, chicken, yeast, human, rat
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... H3F3B(3021)
Related Categories
General description
Specificity
Immunogen
Application
2 μL of this antibody immunoprecipitated chromatin associated with Histone H3 from a wild type yeast lysate.
Quality
Western Blot Analysis:
1:2,000-1:20,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.
Target description
Physical form
Storage and Stability
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Analysis Note
Acid extracted proteins from HeLa cells, HeLa nuclear extracts or acid extracted HeLa cells treated with sodium butyrate.
Other Notes
Legal Information
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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