EPA Method TO-11/IP-6A and ASTM^® D5197: HPLC Analysis of Aldehydes and Ketones on SUPELCOSIL^™ LC-18 after Collection/Desorption using LpDNPH

sample preparation

SPE (Solid Phase Extraction)

adsorbent tube

LpDNPH Cartridge (21024-U)

sample/matrix

5 μg each carbonyl-DNPH on LpDNPH cartridge. Cartridge eluted with 5.0 mL acetonitrile

column

SUPELCOSIL LC-18, 25 cm × 4.6 mm I.D., 5 μm particles (58298)

mobile phase

[A]:acetonitrile:tetrahydrofuran:water, 30:10:60; [B]: acetonitrile:water, 60:40

gradient

gradient 0% B for 1 min, linear gradient to 100% B over 10 min

flow rate

1.5 mL/min

detector

VIS, 360 nm

injection

25 μL of extract