- A spectrophotometric assay for feruloyl esterases.
A spectrophotometric assay for feruloyl esterases.
Analytical biochemistry (2002-10-17)
Vladimír Mastihuba, Lubomír Kremnický, Mária Mastihubová, J L Willett, Gregory L Côté
PMID12381367
ABSTRACT
We have developed a spectrophotometric assay for the quantitative determination of feruloyl esterase activity based on release of 4-nitrophenol from a novel substrate, 4-nitrophenyl ferulate in an emulsion of Triton X-100 in aqueous buffer solution. The release of 4-nitrophenol was linear with reaction time at an early stage of the reaction with various esterase preparations. The method proposed here is accurate, rapid, and easy to perform.
MATERIALS