YEAST1
Yeast Transformation Kit
reagents for introducing plasmid DNA into yeast
Synonym(s):
lithium acetate yeast transformation
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.85
Recommended Products
grade
for molecular biology
Quality Level
usage
kit sufficient for >100 standard transformations
technique(s)
transformation: suitable
shipped in
dry ice
storage temp.
−20°C
Related Categories
General description
Sigma′s Yeast Transformation Kit contains all necessary reagents and controls for efficient transformation of yeast by the lithium acetate method.
Application
Suitable for transformation of any strain of yeast. Convenient, flexible and sensitive, positive transformants can be obtained with as little as 10 ng of DNA; the optimum efficiency is in the 0.1- 3 μg range.
Features and Benefits
- Easy and ready-to-use
- Requires as little as 10 ng of plasmid DNA
- Flexibility for any strain of yeast
- Sufficient for over 100 standard transformations
Components
The Yeast Transformation Kit contains:
- Transformation Buffer; 100 ml; 100 mM lithium acetate, 10 mM Tris HCl, pH 7.6, and 1 mM EDTA
- Plate Buffer; 100 ml; 40% PEG, 100 mM lithium acetate, 10 mM Tris HCl, pH 7.5, 1 mM EDTA
- Deoxyribonucleic acid from salmon teste, 10 mg/ml; 2 x 1 ml
- Control Yeast Plasmid DNA pRS316 carrying the ura gene; 10 μg
- Yeast Synthetic Drop-out Medium Supplement Without Uracil; 1 g
Principle
Transformation with a plasmid complementing the mutated gene enables the transformant to grow on medium lacking the required component. Yeast cells are made competent for transformation by incubation in a buffered lithium acetate solution. Transformation is then carried out by incubating the cells together with transforming DNA and carrier DNA in a solution containing polyethylene glycol (PEG).
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Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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