SAE0005
Glucose-6-phosphate Isomerase human
recombinant, expressed in E. coli
Synonym(s):
GPI, PGI
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About This Item
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recombinant
expressed in E. coli
Quality Level
Assay
≥95% (SDS-PAGE)
specific activity
≥200 units/mg protein
storage temp.
−20°C
Biochem/physiol Actions
Glucose-6-phosphate isomerase (GPI) is a homodimeric enzyme ubiquitously present in most organisms. GPI catalyzes the interconversion between glucose-6-phosphate and fructose-6-hosphate, the second step of the glycolytic pathway. In mammals, Glucose-6-phosphate isomerase also acts as an autocrine motility factor (AMF), a neuroleukin, and a maturation factor. GPI deficiency is the second most common erythroenzymopathy of glycolytic enzymes after pyruvate kinase deficiency. Inherited deficiency of the enzymatic activity of GPI causes hereditary nonspherocytic hemolytic anemia (HNSHA) in humans, a severe deficiency that can be associated with hydrops fetalis (a-thalassaemia), immediate neonatal death, and neurological impairment.
Physical properties
C-terminal histidine-tagged
Unit Definition
One unit will convert 1.0 mmole of D-fructose 6-phosphate to D-glucose 6-phosphate per inute at pH 7.4 at 25 °C.
Physical form
Supplied as a solution in 50 mM Tris-HCl pH 7.5, and 50% glycerol
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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