G8880

Glutaminase from Escherichia coli

Grade V, lyophilized powder, 50-200 units/mg protein

• Synonym(s): L-Glutamine amidohydrolase
• CAS Number: 9001-47-2
• Enzyme Commission number: 3.5.1.2 (BRENDA, IUBMB)
• MDL number: MFCD00131193
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: Escherichia coli
• Quality Level: 200
• type: Grade V
• form: lyophilized powder
• specific activity: 50-200 units/mg protein
• mol wt: 110 kDa
• composition: Protein, ~30% biuret
• cation traces: NH₄⁺: <0.1 μg/unit
• foreign activity: NADH Oxidase ≤0.01%
• storage temp.: −20°C

Description

General description

Glutaminase is an amidohydrolase. It has the catalytic domain in the N-terminal and the C-terminal extension possesses STAS domain.

Application

Glutaminase from E.coli has been used:

• for the determination of glutamine from the cerebral cortex tissue extract
• for immobilization on aminopropylsilylated controlled pore glass (CPG) for determination of glutamine
• in combination with acivicin in in vitro melanoma cells and MCF-7 and OAW-42 cells for cell proliferation and invasiveness assay.

Biochem/physiol Actions

Glutaminase catalyzes the conversion of glutamine to glutamate.

Glutaminase from Escherichia coli along with acivicin, a glutamine analog regulates proliferation and invasiveness of cancer cells. Bacterial glutaminase is implicated in increasing the life span of cancer patients by lowering the tumor burden.

Unit Definition

One unit will deaminate 1.0 μmole of L-glutamine per min at pH 4.9 at 37 °C.

Physical form

Lyophilized powder containing potassium succinate and EDTA

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves