F2426
EZview™ Red ANTI-FLAG® M2 Affinity Gel
clone M2
Synonym(s):
Monoclonal ANTI-FLAG® M2 antibody produced in mouse, Anti-ddddk, Anti-dykddddk
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.32
Recommended Products
clone
M2, monoclonal
Quality Level
analyte chemical class(es)
proteins
technique(s)
affinity chromatography: suitable
immunoprecipitation (IP): suitable
matrix
4% agarose bead; 45-165μm bead size
isotype
IgG1
capacity
≥0.6 mg/mL, gel binding capacity
shipped in
wet ice
storage temp.
−20°C
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General description
EZ view Red Anti-FLAG M2 Affinity Gel is a resin that consists of Anti-FLAG M2 antibody, covalently bonded to 4% Agarose beads. The affinity gel is used to bind FLAG fusion proteins to samples, such as cell lysates and tissue, for purification of FLAG-tagged proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results. Agarose beads bind at N-terminal, Met-N-terminal and C-terminal FLAG fusion proteins, 3xFLAG-tagged fusion proteins.
Specificity
Suitable for purifying N-terminal, Met-N-terminal, C-terminal FLAG fusion proteins, 3xFLAG fusion proteins.
Application
Immunoprecipitation (IP) of FLAG- and 3xFLAG-tagged fusion proteins.
Elution - FLAG peptide, Glycine, pH 3.5, 3x FLAG peptide
Learn more product details in our FLAG® application portal.
Elution - FLAG peptide, Glycine, pH 3.5, 3x FLAG peptide
Learn more product details in our FLAG® application portal.
Physical form
1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
EZview is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
FLAG™ Affinity Gels, FLAG™ tag, 3xFLAG™ tag, DYKDDDDK tag
related product
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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