AFRC 17D1
94120825, Application: Fusion partner for hybridoma production
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About This Item
UNSPSC Code:
41106514
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biological source
Horse x Mouse/Horse Hybridoma
ImmunoDon
Horse peripheral lymphocytes
Myeloma
NS0
Reactivity
Application: Fusion partner for hybridoma production
technique(s)
cell culture | mammalian: suitable
isotype
Not specified
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Horse peripheral blood lymphocytes x Mouse NS0
Cell Line Description
The heterohybridoma AFRC 17D1 was obtained by fusion of lymphocytes from ponies immunised with the influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)) with mouse myeloma NS0 cells. The hybridoma was made aminopterin-sensitive by selective growth in 8-azaguanine. This non-Ig-secreting cell line was used as fusion partner for the establishment of equine-mouse heterohybidomas.
Immunogen
Influenza A equine 2 virus
Application
Fusion partner for hybridoma production
Culture Medium
RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C
Other Notes
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Certificates of Analysis (COA)
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