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Key Documents

14-197-M

Sigma-Aldrich

Casein Kinase 2 Protein, active, 10 µg

Active, recombinant full-length human Casein Kinase 2 protein, containing an α-subunit N-terminal 6His tag & a β-subunit N-terminal GST tag. For use in Kinase Assays.

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.41

biological source

human

Quality Level

100

recombinant

expressed in Sf21 cells

mol wt

α-subunit Mw 49 kDa (heterotetramer; α2β2)
β subunit Mw 53 kDa (heterotetramer; α2β2)

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

NM_001895.3
NM_177559.2
NM_177560.2

UniProt accession no.

P68400

General description

Product Source: Expressed in Sf21 insect cells.
Recombinant full-length human Casein Kinase 2 protein, containing an &alpyha;-subunit N-terminal 6His tag and a β-subunit N-terminal GST tag.

Biochem/physiol Actions

Protein Target: CK2

Packaging

2 vials at 5μg each
Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-197M when ordering the 250μg size.

Quality

Routinely evaluated by phosphorylation of the Casein Kinase 2 Substrate Peptide.

Physical form

20mM MOPS, pH 7.4, 1mM EDTA, 0.1% (v/v) Triton X-100, 0.3% 2-mercaptoethanol, 1mM benzamidine, 50% glycerol.
Sequential chromatography using heparin Sepharose™ and Mono Q anion exchange resin

Storage and Stability

1 year at -20°C.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

Sepharose is a trademark of Cytiva
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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In vitro phosphorylation of the polyomavirus major capsid protein VP1 on serine 66 by casein kinase II
Li, M., et al
The Journal of Biological Chemistry, 270, 26006-26011 (1995)
Casein kinase II beta-subunit inhibits the activity of the catalytic alpha-subunit in the absence of salt.
Tiganis, T, et al.
Biochimica et Biophysica Acta, 1203, 282-289 (1993)
T Tiganis et al.
European journal of biochemistry, 229(3), 703-709 (1995-05-01)
Casein kinase II, cyclin-dependent kinases, and glycogen synthase kinase-3 are members of the protein kinase subfamily with a prominent insert in domain X of their catalytic subunit sequence. The function of the insert sequence in casein kinase II was investigated
Y Fleming et al.
The Biochemical journal, 352 Pt 1, 145-154 (2000-11-04)
Stress-activated protein kinase 1 (SAPK1), also called c-Jun N-terminal kinase (JNK), becomes activated in vivo in response to pro-inflammatory cytokines or cellular stresses. Its full activation requires the phosphorylation of a threonine and a tyrosine residue in a Thr-Pro-Tyr motif

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