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Synthesis of uniform protein-polymer conjugates.

Biomacromolecules (2005-11-15)
Bhalchandra S Lele, Hironobu Murata, Krzysztof Matyjaszewski, Alan J Russell
RESUMEN

We have developed a novel technique to synthesize near-uniform protein-polymer conjugates by initiating atom transfer radical polymerization of monomethoxy poly(ethylene glycol)-methacrylate from 2-bromoisobutyramide derivatives of chymotrypsin (a protein-initiator). Polymerization initiated from the monosubstituted protein-initiator resulted in the conjugate containing a single, near-monodisperse polymer chain per protein molecule with polydispersity index 1.05. Increasing the number of conjugated 2-bromoisobutyramide initiators per molecule of protein increased the molecular weights and polydispersity indices of the final protein-polymer conjugates. The generic nature of this technique was demonstrated by initiating polymerization of nonionic, cationic, and anionic monomers from the protein-initiator. Protein-polymer conjugates synthesized by this novel technique retained 50-86% of the original enzyme activity. The technique described herein should be useful in synthesizing well-defined protein-polymer conjugates exhibiting a wide range of physical and chemical properties.

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Sigma-Aldrich
Butyramide, ≥98.0% (T)
Sigma-Aldrich
2-Bromoisobutanoic acid N-hydroxysuccinimide ester, 98% (GC)