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Merck

Responses of the L5178Y tk+/tk- mouse lymphoma cell forward mutation assay to coded chemicals. I: Results for nine compounds.

Environmental mutagenesis (1987-01-01)
D B McGregor, R Martin, P Cattanach, I Edwards, D McBride, W J Caspary
RESUMEN

Nine substances were tested for their mutagenic potential in the L5178Y tk+/tk- mouse lymphoma cell forward mutation assay, by means of procedures based upon those described by Clive and Spector (Mutat Res 44:269-278, 1975) and Clive et al (Mutat Res 59:61-108, 1979). Cultures were exposed to the chemicals for 4 hr, then cultured for 2 days before plating in soft agar with or without trifluorothymidine (TFT), 3 micrograms/ml. The coded chemicals were tested at least twice. Significant responses were obtained with calcium chromate, 3-(chloromethyl)pyridine, 1,2-epoxybutane, monochloroacetic acid, dicyclohexylthiourea, 2,4-diaminophenol hydrochloride, and pentachloroanisole. Apart from pentachloroanisole, rat liver S9 mix was not a requirement for the clearly mutagenic activity of any of these compounds. Compounds not identified as mutagens were 3-amino-1,2,4-triazole and sucrose.

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Sigma-Aldrich
1,2-Epoxybutane, 99%
Sigma-Aldrich
3-(Chloromethyl)pyridine hydrochloride, 96%
Sigma-Aldrich
(R)-(+)-1,2-Epoxybutane, 98%
Sigma-Aldrich
(S)-(−)-1,2-Epoxybutane, 98%