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Merck

Look and see if it is time to induce protein expression in Escherichia coli cultures.

Biochemistry (2010-06-15)
K Danielle Kelley, Lorenzo Q Olive, Arina Hadziselimovic, Charles R Sanders
RESUMEN

It is shown that Methyl Red can be used as an indicator dye that changes color in Escherichia coli culture as a result of time- and cell density-dependent bleaching by azoreductase produced by the bacteria. For cell cultures that are being used to express a recombinant protein, this phenomenon can be exploited to provide a simple visual cue that cell cultures have reached an appropriate growth phase for addition of an agent to induce protein expression, such as isopropyl thiogalactoside.

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Sigma-Aldrich
Methyl Red, ACS reagent, crystalline
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Methyl Red solution, suitable for microbiology
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Methyl Red sodium salt, ACS reagent, Dye content 95 %
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Methyl Red sodium salt, Crystalline
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Methyl Red, JIS special grade
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Methyl Red solution, pH 4.2-6.2