Saltar al contenido
Merck

Identification of biochemical and putative biological role of a xenolog from Escherichia coli using structural analysis.

Proteins (2011-02-05)
Varun Bhaskar, Manoj Kumar, Sankar Manicka, Sunil Tripathi, Aparna Venkatraman, S Krishnaswamy
RESUMEN

YagE is a 33 kDa prophage protein encoded by CP4-6 prophage element in Escherichia coli K12 genome. Here, we report the structures of YagE complexes with pyruvate (PDB Id 3N2X) and KDGal (2-keto-3-deoxy galactonate) (PDB Id 3NEV) at 2.2A resolution. Pyruvate depletion assay in presence of glyceraldehyde shows that YagE catalyses the aldol condensation of pyruvate and glyceraldehyde. Our results indicate that the biochemical function of YagE is that of a 2-keto-3-deoxy gluconate (KDG) aldolase. Interestingly, E. coli K12 genome lacks an intrinsic KDG aldolase. Moreover, the over-expression of YagE increases cell viability in the presence of certain bactericidal antibiotics, indicating a putative biological role of YagE as a prophage encoded virulence factor enabling the survival of bacteria in the presence of certain antibiotics. The analysis implies a possible mechanism of antibiotic resistance conferred by the over-expression of prophage encoded YagE to E. coli.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
N-Acetylneuraminic Acid Aldolase from microorganisms, lyophilized powder, ≥20 units/mg protein (biuret)